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Article XI

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Additional data regarding pathogen cells I. Labelling with gold complex probes II. Occurrence of electron opaque bodies (EOBs) in fungal cells III. Other fungal endo-elements IV. More about components in host tissue of probable patho
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In this article, titles of the five parts forming it are considered to well indicate their content. Briefly, labelling results (part I) of pathogen elements provide indications on their possible stages of development regarding their cell walls and content, their relation with extracellular matter and/or mode of action. Concerning EOBs (part II), their appearance may at times be analogous to endocells (part III) and perhaps represent a kind of quiescent or reproductive element of the fungus involved. An interesting feature in this respect was the observed outstanding number of bodies in cells of O. novo-ulmi  growing on Millipore membranes and a medium to which was added some BRDU, which apparently resulted from modified mitochondria. More is presented (part IV) about the occurrence and structure of opaque matter in the form of large bodies in middle lamellae or in cells as well as of some extending along vessel walls, and of their links with fungal elements. Concerning filtrates of O. novo-ulmi  cultures passed through Millipore membranes, bodies from which the pathogen could have developed in a number of cases are illustrated, as well as the colonies formed from the plated filtrate samples, many being waxy-like and yielding typical cultures only following transfers from these colonies. The presence of the fungus in these filtrates was also verified by its producing typical coremia on inoculated sterilized wood blocks.
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